Speed-oligo® is designed to bring Molecular Biology techniques into laboratories of diagnosis of infectious diseases through a very simple technique. Speed-oligo® combines all the advantages of Molecular Biology in a rapid test. Any laboratory with minimal equipment can introduce the PCR technique, which provides automated results in just 5 minutes. Laboratories that use this technique through “home-made kits” can also benefit from the high reproducibility, sensitivity and specificity provided by the Speed-oligo® commercial kit without forgetting the advantages of chromatography revealing. Even clients using RT-PCR may be interested in Speed-oligo®. Vircell offers “exotic” parameters that require a low number of determinations, making Speed-oligo® more interesting than RT-PCR in terms of price and equipments expiration.
It is possible to assay the Speed-oligo® technique with just a thermocycler (conventional or fast). These instruments are very common in Molecular Biology laboratories and do not need to be used exclusively for this technique.
As with any other PCR technique, Speed-oligo® is assayed with nucleic acid. Each Speed-oligo® reference needs a different type of sample from which the nucleic acid is extracted. For some of the kits, respiratory samples are used (Bacterial pneumonia combi, Chlamydophila pneumoniae, Legionella pneumophila or Mycoplasma pneumoniae) while for other kits, cephalorraquid fluid is used (Bacterial meningitis), vaginal samples or rectovaginal (Group B streptococcus) or culture (Mycobacteria).
Speed-oligo® does not require special conditions for transport. As this product includes the positive control and the PCR-mix in lyophilized presentation, the transport can be done at room temperature. The product should be stored in a refrigerated container at temperatures between 2 and 8ºC.
All Speed-oligo® products have a 12 months shelf life.
Even though Speed-oligo® is a technologically advanced product, its price policy is to position itself as a Molecular Biology technique affordable for any kind of laboratory. In the price analysis made before launching the product into the market, the price of Speed-oligo® turned out to be 2 to 4 times lower than gPCR and other similar techniques on the market, and a bit higher than conventional PCR. Thus, Speed-oligo® offers the reproducibility advantages of a ready-to-use commercial kit and is very simple to use as well.
The Speed-oligo® cassette contains lyophilized PCR mix and a disposable cassette including test strip. The user just needs to resuspend the prepared PCR mix and add the clinical sample.
The new design is a closed, self-contained cassette allowing for a contamination-free protocol.
We recommend using this technique in a Molecular Biology laboratory with well differentiated pre- and post-amplification areas.