Speed-oligo® aims to bring Molecular Biology techniques closer to laboratories for the diagnosis of infectious diseases offering a very simple technique. It combines all the advantages of Molecular Biology in a quick test.
This technique is suitable for:
• Diagnostic laboratories wishing to incorporate Molecular Biology techniques into their routine work.
• Laboratories that use PCR techniques and need a quick and simple detection method with maximum reproducibility, sensitivity and specificity.
• Laboratories in emergency services.
• Laboratories in reference centers that are looking for a commercial alternative to homemade PCR.
• Even customers using qPCR may be interested in Speed-oligo®. In the "exotic" parameters offered by Vircell, the small number of determinations makes Speed-oligo® more interesting than qPCR in terms of cost and expiration of equipment.
With only a thermocycler (conventional or fast) and a thermoblock it is possible to perform the Speed-oligo® technique. These instruments are very common in Molecular Biology laboratories and do not need to be used exclusively for this technique.
As with any other PCR technique, Speed-oligo® is assayed with nucleic acid. Each Speed-oligo® reference needs a different type of sample from which the nucleic acid is extracted. For some of the kits, respiratory samples are used (Bacterial pneumonia combi, Chlamydophila pneumoniae, Legionella pneumophila or Mycoplasma pneumoniae) while for other kits, cerebrospinal fluid is used (Bacterial meningitis), vaginal samples or rectovaginal (Group B streptococcus) or culture (Mycobacteria).
Regarding the extraction of the samples, Vircell offers reagents to carry it out in the determinations of Streptococcus group B and mycobacteria. These references include the VIRCELL SAMPLE SOLUTION reagent, which by mixing it with the sample and heating it at 95°C, allows obtaining quality DNA to be amplified by PCR. However, it is not possible to use this extraction method with other Speed-oligo® references since the cerebrospinal fluid and other non-respiratory samples contain a large number of inhibitors that must be eliminated with a specific extraction kit. For these cases, any commercial kit, manual or automatic, can be used (Qiagen, Roche, etc.).
Speed-oligo® has been tested with different extraction kits: High pure PCR template preparation kit (Roche), QIAamp DNA blood mini kit (Qiagen) or NucleoSpin tissue (Macherey-Nagel). However, other commercial equipment can also be used.
In any case, the references for the detection of Streptococcus group B and mycobacteria (DMT only for respiratory samples) include the VIRCELL SAMPLE SOLUTION reagent for DNA extraction.
SPEED-OLIGO® MYCOBACTERIA has only been validated for identification in culture samples since culture remains the reference technique in the mycobacterial laboratory. Even so, the identification of positive cultures remains a challenge, since the existing methods are slow and require a lot of technology. SPEED-OLIGO® MYCOBACTERIA, for its simplicity and speed, is a perfect complement for mycobacteria culture since it allows a qualitative detection of the Mycobacterium genus and 19 different species of Mycobacteria.
If a direct sample detection is preferred, SPEED-OLIGO® DIRECT MYCOBACTERIUM TUBERCULOSIS can be used to distinguish between MTB and MOTT without the need to wait for the culture.
As reflected in the instructions for use included in the product, the sensitivity of this product is 98% (100% in MTB) with 100% specificity. In the 19th edition of ECCMID (European Congress of Clinical Microbiology and Infectious Diseases) that took place in Helsinki, the following work was presented: "Multi-center evaluation of Speed-oligo Mycobacteria version 2 assay for identification of Mycobacterium spp in routine clinical mycobacteriology" (Martínez-Lirola, MJ et al., 2014). This study concluded that SPEED-OLIGO® MYCOBACTERIA is able to efficiently distinguish among the most frequent species of mycobacteria. The reference of this publication is available in the Bibliography section of our website.
Speed-oligo® does not allow an identification as exhaustive as that achieved by sequencing. However, it brings cost-benefit advantages.
Speed-oligo® does not require special conditions for transport. As this product includes the positive control and the PCR-mix in lyophilized presentation, the transport can be done at room temperature. The product should be stored in a refrigerated container at temperatures between 2 and 8ºC.
Speed-oligo® products have an 18 months shelf life from the date of manufacture.
Even though Speed-oligo® is a technologically advanced product, its price policy is to position itself as a Molecular Biology technique affordable for any kind of laboratory. In the price analysis made before launching the product into the market, the price of Speed-oligo® turned out to be 2 to 4 times lower than qPCR and other similar techniques on the market and a bit higher than conventional PCR since it offers the advantages of a closed commercial kit such as reproducibility and simplicity.
We recommend using this technique in a Molecular Biology laboratory with well differentiated pre- and post-amplification areas.