Brucellacapt® uses the Brucella abortus strain although, serologically, there is no difference between Brucella abortus, Brucella melitensis and Brucella suis. According to the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals from the OIE (http://www.oie.int/eng/normes/mmanual/A_00052.htm): "It should be emphasised that antigen made with one of these B. abortus strains is also used to test for B. melitensis or B. suis infection. According to the Manual of Clinical Microbiology, 8th ed from the ASM: "The standard SAT method uses killed whole-cell B. abortus antigen and detects antibodies against B. abortus, B. suis and B. melitensis but not B. canis."
According to WHO, the only way to distinguish between B. abortus and B. melitens is through bacteriological culture (isolation of the bacteria from samples). Brucellacapt®, like the other current serological methods (ELISA, Rose Bengal, SAT), will not discriminate between Brucella abortus, Brucella melitensis or any of the strains that contain LPS, which is common to all species of Brucella.
Although treatment of the disease is not Vircell’s field of expertise, scientific literature supports that the treatment for both infections is the same (both are sensitive to the same antibiotics) with the exception of some rare strains such as B. melitensis strain REV-1.Therefore, differentiation is only relevant from the epidemiological point of view only. Using Brucellacapt® offers the additional advantage (compared ELISA, SAT, Rose Bengal etc…) that it allows for monitoring the evolution of the patient during their treatment, and it allows the early detection of reinfections and chronic phases of the illness. In any case please refer to the recommendations published by the WHO for diagnosis and treatment of brucellosis, for more specific information.
Brucellacapt® can be used in two ways: in titration (24 tests), or in screening (96 tests). Both procedures are explained in the package insert. The kit includes enough diluent to perform titration. To use it in screening mode, additional serum diluent will be required (Ref. B0002). This can be acquired separately through your local distributor. In both cases, it is highly recommended to use of a humid chamber during incubation time, so as to avoid desiccation of the sample. Vircell can provide you with humid chambers, free of charge, on request.
The SAT test refers to Wright’s Serum Agglutination Test. Rose Bengal is a rapid agglutination test. Also, the acidic medium in which Rose Bengal is performed makes it more sensitive and specific in comparison to SAT.