VirClia
A VirClia® kit contains 24 single-use tests and includes all the necessary reagents to perform the test. Each strip is composed of 3 reaction wells and 5 reagent wells.
- VIRCLIA WASHING SOLUTION: 4 x 50 ml of washing solution (20x concentrated): phosphate buffer with TweenR-20 and with Proclin.
- VIRCLIA DECONTAMINATION SOLUTION: 3 x 20 ml of 0.5 M sulfuric acid solution.
These components are generic. The use of these auxiliary reagents is recommended to guarantee the best performance of the tests.
VirClia® kits expire 15 months after their manufacture date, if the storage conditions (2-8ºC) are complied with.
VirClia® kits include all the necessary reagents to perform the test. However, we also offer an auxiliary reagents kit (ref. VCMAR) for washing and decontamination purposes. Generally, you will need 1 “VIRCLIA® AUXILIARY REAGENTS” Kit for every 8 test kits.
10 reusable frames are provided with each machine. If extra frames are needed, they can be purchased using the reference (VCFRAME). The replacement of frames is recommended if they warp after prolonged use.
Yes, since it is an open and dual system (ELISA / CLIA), both techniques can be performed for infectious diseases and for other disciplines (autoimmunity, allergies, etc.).
A maximum of 24 samples (2 plates) can be analyzed in each VirClia® assay. However, the sample racks can hold up to 192 sample tubes that are used in ELISA assays.
Yes, the assay is completely customizable. All VirClia® tests, with the exception of avidity tests, are compatible with one another and can be combined in the same process.
All indirect tests are identical since sorbent is included in the IgM strips. However, capture, quantitative, avidity and sample pre-dilution tests include modifications or extra steps. In any case, all VirClia® tests, apart from avidity tests, are completely compatible with one another and can be combined in the same process.
If 1-3 strips are placed in the frame, the test lasts approximately 50 minutes. One plate (12 strips) lasts about 1 and a half hours, and two complete plates (24 strips), about 2 hours.
No. Each strip includes a calibrator and a negative control that allows the validation and interpretation of the results for each individual sample and test. It is not based on stored curves.
The machines intended to process the VirClia® tests have a dual ELISA/CLIA reader installed, so the customer will receive the two software, one for each technique. In addition, the system will include a hardware testing software (Testing Tool).
The loading wizard will guide you through the process of loading strips. The strips are grouped by tests and alphabetically to facilitate this process. Some techniques include prefixes in the name of the testfile that must be respected. This is because they must be processed at the beginning or at the end of the test, and the modification of the prefix would alter the order of processing, and may influence the results.
Yes, VirClia® and ELISA software are integrated with most LIS. In addition, Vircell has developed its own middleware, VirCom, to facilitate the interaction between the VirClia® system and the LIS, simplifying the user's routine.
The needles are washed with washing solution after each pipetting step and a decontamination solution (0.5M sulfuric acid) is used as an additional washing step after conjugate pipetting. Vircell has carried out qualitative and quantitative tests (available on request) that ruled out the carry-over between samples, between sample and calibrator, and that associated with the conjugate.