DENGUE ELISA
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Indirect immunoenzyme assay to test antibodies against dengue virus in human serum/plasma.
- Serum dilution and sorbent treatment, when necessary, directly in the well
- The M1018 reference is based on µ-Capture technique
- Suitable for automated ELISA systems
PRODUCT | REF. | Nº TEST | CLASS |
---|---|---|---|
PRODUCT:
DENGUE ELISA IgG
|
REF.: G1018 | Nº TEST: 96 |
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PRODUCT:
DENGUE ELISA IgM CAPTURE
|
REF.: M1018 | Nº TEST: 96 |
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Indirect Method - The ELISA method is based upon the reaction of antibodies in the sample tested with the antigen adsorbed on the polystyrene surface. Unbound immunoglobulins are washed off. An enzyme-labelled anti-human globulin binds the antigenantibody complex in a second step. After a new washing step, bound conjugate is developed with the aid of a substrate solution (TMB) to render a blue coloured soluble product which turns into yellow after adding the acid stopping solution.
Capture Method - The ELISA method is based upon the capture of IgM in the sample with anti-IgM antibodies adsorbed on the polystyrene surface. Unbound immunoglobulins are washed off. Then the antigen labeled with peroxidase react with the IgM captured, and the unbound is eliminated by washing; bound antigen is developed with the aid of a substrate solution (TMB) to render a blue coloured soluble product which turns into yellow after adding the acid stopping solution.
- High performance and guaranteed stability with lyophilized conjugates when necessary.
- Samples and controls are equally processed to compensate pipetting variability.
- Colour-coded plates with individual break-apart wells.
- Coloured, ready-to-use liquid reagents.